How to Use a Microscope

Compound Microscopes

1. Click the lowest power objective lens (e.g. 4x) into position by rotating the revolving turret (2).
   
2. The microscope slide should be placed on the stage (6) and fastened with the stage clips.
3. Turn the focus knob (4) so the stage moves upward as you look at the objective lens (3) and the stage from the side. Move it as high as possible without letting the objective touch the coverslip.
4. By moving the focus knob, the image will come into focus when you look through the eyepiece (1).
5. Adjust the condenser (7) and light intensity to achieve the maximum amount of light.
6. Make sure the sample is in the center of the field of view (what you see) by moving the microscope slide around.
7. The focus knob (4) is used to focus the sample, and the condenser (7) and light intensity are adjusted for the clearest image (if you are using low power objectives, you may need to reduce the light intensity or close the condenser).
8. With the lowest power objective, you can switch to the next objective once the image of your sample is clear. You might need to adjust the focal point of the sample and/or the light intensity of the condenser. Using the higher power objective lens, repeat steps 3 through 5 if you are unable to focus on your specimen. Keep the objective lens away from the slide at all times!
9. Slide the low power lens into place, lower the stage, and remove the slide.

"Your microscope slide should be prepared with a coverslip over the sample to protect the objective lenses if they touch the slide."

NOTES:

• You should not touch the glass part of the lenses with your fingers. Clean the lenses only with special lens paper.
• When not in use, cover your microscope.
• Carry a microscope with both hands at all times. Place one hand under the base of the arm and the other under the arm.

Stereomicroscopes

1. Turn on the LED light (2) and place your sample on the stage (3).
2. Move the focus knob (1) until the image comes into focus through the eyepieces (4).
3. You should be able to see the sample clearly with both eyes simultaneously by adjusting the distance between the eyepieces (4).

NOTES:

• The focus knob will need to be moved again if you move the sample.